AHRI report No.38

Diagnosis of Animal Pathogens by Electron Microscopy Techniques

National Institute for Animal Health, Council of Agriculture, Executive of Yuan 

 In this report, unique morphology and sizes of pathogens are observed by TEM technique and used as a basis for preliminary diagnosis of animal diseases. The negative staining technique contains advantages of simplified treatment and improved TEM observation, providing improved efficiency for diagnosis. This report used negative staining technique to examine 1,641 samples collected from poultry, ruminants, aquatic animals, pigs and dogs.  Furthermore, 9 out of the 1,641 samples were applied to electron microscopy with ultrathinsection examinations, and 3 were applied to the immunogold examinations. We observed 15 families of virus including Paramyxoviridae, Orthomyxoviridae, Rhabdoviridae and  aliciviridae, Nodavirus. These virus pictures are archived as references for future references. 

 Ultrathinsection, negative stain, immunogold examination, animal disease