| Reportno. |
AHRI report No.42 |
| Topic |
Development of Molecular Diagnostic Methods for Detecting West Nile Virus in Wild Birds in Taiwan in 2006
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| Department |
1Animal Health Research Institute, Council of Agriculture, Executive Yuan
2 Centers of Disease Control and Prevention, United States
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| Author |
Liu YP1*1, Chang GJ2, Cheng MC1, Lee MS1, Chen LH1, Lee SH1
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| Summary |
West Nile virus (WNV) infection is an important zoonosis. To prevent the possible introduction of this emerging disease into Taiwan , we have established a comprehensive wild-bird surveillance system for monitoring the presence of West Nile viral RNAs in migratory birds. Three detection methods for West Nile viral RNA, including nested PCR, SYBR Green I real-time PCR and Multiplex TaqMan real-time PCR, were used for this study. The nested PCR assay could detect 5 RNA copies, and it was 1000-fold more sensitive than that of traditional PCR. The sensitivity of SYBR Green I real-time PCR assay was equal to nested PCR. However, if the standard curve generated from known copy-number control specimens was included in the assay, the SYBR Green assay could be used to quantify the copy number of testing specimens. The multiplex TaqMan real-time PCR could differentiate four important flaviviruses, including WNV, JE, SLE and YF viruses. We have incorporated the surveillance project of WNV into the avian influenza surveillance project established previously. A total of 4,626 specimens from wild birds were processed in 2006, and none of them was WNV positive
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| Keyword |
West Nile fever, Wild birds, Real-time polymerase chain reaction
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