| Summary |
We have applied histopathology, immunohistcchernistry, Western immunoUot assay, and enzyme-linked imm nc6orlrnt assay technirpies in the continuous surveillance gogram of bonne
spgifonn encephalopathy (ESE). Among & 859 cattle mains, 826 mains were coflerti randomly from the alattoirs, 3 from the M. tukerculcths-contaminated farms, 6 from the sick cows imjrorted from Canada, 1 from the bo’vine ephemeral fever virus infected cattle and 23 from the culling old cattle in Taiwan in 2(01. Cattle develored central nervous signs ci- showed other symptoms were also included. All of the brains were negative. Additionally, histopathological obrervation and direct
ulLlInary immunofluoxescent antibcdy were also applied to examine 1 rabies-suscaDted case with nervous symptom and 79 brain tissues of stray dogs in 2(01. Rabies viral antigens and typical lesion were not detuied in all tissues indicating fr stutus of rabies in Taiwan. On the other hand, 3,204 sera samples inclurli jig 1,380 samples from domestic dogs and 1,821
samples from stay dogs were examined by the enzyme-linked irnmunosorkent assay. Results showed that the sero-pcsitive rate of domestic and stray dogs were 5 8.6% (80 ./1 ,3 80) and 35.9% (654/1,821), restively.
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