| Summary |
An analysis of residue of tiamulin in pork by microbiological assay with cylinder plate method was established. Tiamulin was extracted with methanol, cleaned up with n-hexane after centtifugation and then condensed. The sediment was dissolved in phosphate buffer of pH 8.0 ± 0.1, and poured into cylinder on medium cultured with Staphyloccoccus auseus for detection of residual level of the drug. The recoveries were in the range of 58.6 % to 14.8 % spiked with 0.1 to l.Opag: g of tiamulin in pork, and the coefficient of variances were all below 6 %. The detection was 0.05ag g.
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