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Summary
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Classical swine fever virus is the most important pathogen for damaging the immune system for its high affinity to the immune cell, resulting in leucopenia, and lymphocytopenia. The project is aimed to compare the immune response effects of pigs vaccinated with the frozen dried lapinized hog cholera vaccine and the E2 subunit vaccine. The results showed that there was no significant difference in clinical observation, including body temperature, appetite and spiritual. The results of blood exams, such as white blood cells and red blood cells distribution were similar among groups. We observed that the neutralizing antibody against hog cholera virus began to appear on 14th day, and showed all positive on 23rd day post vaccination in both the frozen dried lapinized hog cholera and E2 subunit groups. In addition to the similar ratio of CD 4, CD 8, IgM, and macrophage concentrations in the blood, no differences revealed in the results of analysis of peripheral blood mononuclear cells (PBMC) and other components in the blood as compared the control and vaccinated groups. In addition, the results show that detection of blood IL-1, IL-4, GM-CSF and TNF-α concentrations in each group have no significant difference; detection of IL-6 concentrations in blood in the frozen dried lapinized hog cholera vaccine group was increased significantly on 4 days and 7 days; detection of blood concentrations of IL-12 in each group on 1 day was significantly increased; detection of IFN-γ concentration in blood in the E2 subunit vaccine group was increased significantly on 2 days, and the frozen dried lapinized hog cholera vaccine group on 3 days was also increased significantly. Different vaccines will produce different cytokines, and whether it has the ability to activate other immune cells, or other regulatory pathways to regulate antibody production? More experiments will be further confirmed.
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