| Reportno. |
AHRI report No.41
|
| Topic |
Development of avian influenza subtype-specific reference antisera by DNA vaccination
|
| Department |
Animal Health Research Institute, Council of Agriculture, Executive Yuan |
| Author |
Lee MS*, Chen LH, Liu YP, Cheng MC, Lee SH |
| Summary |
The HA1 subunit of the H5 gene was amplified by reverse transcriptase / polymerase chain reaction (RT-PCR) and cloned into the PCR-Ⅱ Blunt Topo vectors (Invitrogen ). Then the HA1 gene was subcloned into the pCI-neo vector（Promega,Madison, WI） and pCDNA3.1 vector (Invitrogen ). Six-week-old BALB/c mice and three-week-old specific pathogen free (SPF) chickens were vaccinated with 100 µg of eukaryotic expression vectors mixed with a liposome by intramuscular injection. Then the animals were boostered twice at monthly intervals after the first vaccination. The HI antibody titers were low.
|
| Keyword |
Avian influenza,DNA vaccine,Specific antibody
|