| Summary |
The public health problem resulted from the commonly use of the carbadox (CBX ) in swine feed has spurred so many scholars trying to develop a simply and precisely detecting method. The most difficult prob-lem was the preparatim of feed sample. We extracted the CBX from the feed by pretreating with water, extracting with 95% dimethylformamide overnight at roan temperature, cleaning up on ·a colunnn of alwnina and filtering thrrugh a 0.45u millpore filter membrane. Injected 20 ul filt-erate into high performance liquid chromatography. The mobile phase was acetonitrile mixed with double distilled water ( 20+80) and its flow rate was 2.5 ml /min. The chromatogram showed excellent baseline resol-ution, and no interfering peak. Recoveries from medicated feed were 91.2 % for 50 ppm, 93.8% for 10 ppm and 92.5% totally. This method was capable of detecting from 2 to 0.2 ppm for CBX standard concentration. Its repeatabilities were 96.7% for peak area and 99.5% for retention time. The wavelength of maximum absorbance of CBX was 313 run accord-ing to spectrophotometry. These results suggested the possibiIity of a simple and precise method for detecting the CBX in feed.
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