| Summary |
A monoclonal antibody capture enzyme-linked immunosorbenl assay (mAb-ELISA) for antibodies to infectious bursa! disease virus ((BOY) in chicken sera was developed and compared with conventional ELISA. When sera from farm chickens were tested by the two ELISAs and serum neutralization (SN), the correlation rate between SN and mAb-ELISA was 100% (49/49), and that between SN and conventional ELISA was 81.6% (40/49). In mAb-ELISA, all of the sera that were antibody-negative by SN had low absorbance values (below 0.05), and the absorbance values correlated closely with the SN tilers. In the conventional ELISA, however, the scra antibody-negative by SN had various absorbance values ranging from 0.06 lo 0.32. mAb-ELISA had much lower non-specific reactions than the conventional ELISA against sera from IBO-negative chickens.
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