AHRI report No.24

Investigations on the monensin residuses in the ment and egg of chicken fed on the monensin-containing feed. (1) Studies on the methods for the detection of monensin in meat and egg of chicken.

Animal Health Researdi Institut Cc’uncil of Agriculture

The layers and list were fern with 1(I) ppm nonensin. Egg liver and meat of broilers were coDect every 5 day suentially and extract by caxbDn tetachlori&. The extin were eluated with chloroform-rnethanol(95:5) in silica gel column. The elu were concentrated and duel. The residues were dissolvel in methanol and appliel on thin layer chromatography plate and developped with ethylacewe:igooctane:acetic acid:t-iethylamine (2fX):6:0.4:0.2). Thc€e plates ndrielandanalyby bicautography with Baciiilus subtilis (ATOD 6633), These result indicatul the detution limit was more them25 ppb. The rovey rate from egg and meat were 63% and 70%, receptivity.

monensinBacilllus subtilis zhicken