Samples collected from both CMT-positive milk and the environ-ments were used for bacterial isolation. The major pathogens in milk were streptococcus agalactiae (49.4%) and Staphylococcus spp (33.9%). On the other hand, the distribution of the latter was mainly (40.0%) in farms in which the poor sanitation was maintained and the old milking machine was used. The major distributions of Strep-tococcus spp and staphylococcus spp in enviornments were found in teat caps, milk sucker tube and milk Pool tank. The major bacteria on cow's body surface farm floor, feed trough and water trough were Escherichia coli (38.4%), Klebsiella spp (27.5%) and a few Staphylococci.