Detection of Dual-infection of Porcine Circovirus Type 2 and Torque Teno Sus Virus in Taiwan

C Wang1*, TS Huang1, SH Lee2, JC Chang1, YL Lin1, HJ Tsai1

1Animal Health Research Institute, Council of Agriculture, Executive Yuan

2Animal Drugs Inspection Branch, Animal Health Research Institute, Council of Agriculture, Executive Yuan

Abstract

Porcine circovirus type 2 (PCV2) is a prominent swine viral pathogen. PCV2 is considered as the major causative agent of PCV2 associated disease (PCVAD). Co-infection of PCV2 with other viral pathogens is common; one of which is the Torque teno sus virus. Torque teno sus virus (TTSuV) contains 2 major genotypes, TTSuV and TTSuV2, and is known to have a high prevalence in pig populations throughout the world. The purpose of this study was to investigate the prevalence of co-infection of PCV2 and TTSuV in Taiwanese pig farms. A total of 243 clinical tissue samples collected from pigs between years 2011 and 2014 were used. The PCV2 and TTSuV sequences were amplified and differentiated by using the 1oop mediated isothermal amplification (LAMP) and the nested polymerase chain reaction (nested PCR), respectively. Of the 243 clinical samples, 93.4％ were PCV2 positive, 6.1％ were TTSuV1 positive alone, 8.2％ were TTSuV2 positive alone, and 83.5％ were TTSuV1and TTSuV2 double positive. The rate of co-infection of PCV2 and TTSuV1, PCV2 and TTSuV2 as well as PCV2 and TTSuV1/TTSuV2 were 6.1％, 8.2％, and 78.6％, respectively.

Keywords: Porcine circovirus type 2, torque teno sus virus, loop mediated isothermal amplification, nested polymerase chain reaction.