Cultivating three bacterial strains, Rimerella anatipestifer、Escherichia coli and Pasteurella multocida respectively in broth medium through shaker incubator and fermentor, so as to find the best culture conditions for them and replace conventional ways in agar medium. Then to improve defects of investing lots of labor and space in large scale production, produce high titer、high antigenicity and economical bacterial vaccine eventually.