| Reportno. |
AHRI report No.41
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| Topic |
Introducing and application of the automated system to animal health inspection and quarantine
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| Department |
Animal Health Research Institute, Council of Agriculture, Executive of Yuan |
| Author |
Chang JC*, Lee SH, Chang KH, Tsai KR, Lee MS, Ting LJ, Chen YP |
| Summary |
We have set up the real-time PCR machine and the procedure of the nucleic acid detection with real-time polymerase chain reaction (PCR). The procedure was applied to detect canine parvovirus firstly. The level of nucleic acid which could be detected by the constructed assay in this study was as low as 1 pg. The standard curve about the relationship between concentration of nucleic acid and cycle numbers could be applied to quantify the nucleic acid. Information provided from Indiana Animal Disease Diagnostic Laboratory (ADDL) and Kentucky Livestock Disease Diagnostic Center (LDDC) can be applied successfully to set up an automated information system for animal inspection and quarantine in the future.
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| Keyword |
Real-time PCR, Automated information system, Animal health inspection and quarantine
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