| Summary |
The FLK cells with bovine leucosis virus (BLV) persisting infection were cultured in Hank’s base growth solution (containing 10% fetal calf serum and 0.04 mg/mL gentamycine, used for preparing the A antigen) and in basal medium supplement growth solution (Biochrom KG, used for preparing the B antigen). The supernatants were harvested, inactivated, concentrated, and prepared for antigen production. Both the A (A-Ag) and B (B-Ag) antigens were used for the agar gel immunodiffusion (AGID) test and Enzyme-linked immunosorbent assay (ELISA). A total of 746 bovine sera collected from 21 prefectures were tested with the A-Ag and B-Ag in the AGID test. Results indicated that the positive rates were 38.74% (289/746) for the A antigen and 39.41% (294/746) for the B antigen. In ELISA, the coating antigen was prepared from B antigen fractionated with Sephacryl S-300. The
ELISA-plates were coated with the purified B antigen for overnight at 4℃. Serum samples from 7 prefectures were assayed for anti-BLV antibodies with ELISA and
AGID test. Results showed that positive rates were 38.84% (394/757) for ELISA and 36.2% (274/757) for the AGID test.
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