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Update Date: [2022-09-30]

Publication Polymerase Chain Reaction for Diagnosis of Hog Cholera Carriers

Reportno.

AHRI report No.31

Topic

Polymerase Chain Reaction for Diagnosis of Hog Cholera Carriers

Department

Taiwan Animal Health Research Institute.

Author Lin, Y. P., * N. J. Li, Y. L. Lin, K. F. Lin, T. S. Hung and Y. H. Yang 
Summary

The serum samples of sows and young pigs were collected from the areas of Pintung, Koashiung, Tainan and Changhuan, that farms had HC occered more then one year ago. The highest titer against HC was 1 : 1024 in Pintung. 

Next year, a totle of 138 serum samples were collected from low titer of sows and tested the HC titers again. The results were still low. It's ranging were between 1 : 2~ 1 : 64, the average ranging was 1 : 8. 

To survey the invasion of chronic or clinically healthy carriers or subclinical hog cholera infection, a totle of 143 samples were collected from 7 highest and 6 lowest titer sows. The testings include (1) polymerase chain reaction (2) inoculate STY or CPK cells (3) FA test. The results suggest that no HCV could be detected. 

To survey the invasion of Hog Cholera Carriers, a totle of 138 swab samples were collected from low titer of sows. The testings include (1) polymerase chain reaction (2) inoculate STY or CPK cells(3)  FA test. The results suggest that no HCV could be detected. Next, a totle of 1440 swab samples were collected from pigs and sows of A farm for every month. The results suggest that no HCV could be detected too .For the HC titers, the serum samples of 720 sows were tested by END method. It's ranging were between 1 : 8~ 1 : 384, the average ranging was 1 : 32. 
The swab samples collected from the farm that occurred HC after one month there could be detected the HCV from pigs that titer below 8X.

Keyword

Polymerase Chain Reaction;Hog cholera; carriers.

 


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