For detecting swine vesicular disease (SVD) antigen, irnmunofluorescence technique was recommf!nded. However, both-double immuno-diffusion(DID)and serum neutralization test (SNT) could be also applied for the diagnosis and serological survey of SVD. DID was less sensitive than SNTin detecting SVD antibody. DID were performed in parallel with SNTin a total of 131 serum samples. 49 of 131 sera (37.4%) showed positive in DID. None of 20 sera with serum neutralization titers equal or lower 1 : 4 was positive in DID. Conversely, none of 36 sera with serum neutralization titers of 1 : 128 or higher was negative in DID.