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The production of pseudorabies virus from RK-13 monolayer cells grown on the surface of cytodex I microcarriers and the potency of the vaccines prepared with such virus were investigated. The titer of the virus was 109.0 TCID50/ml. The virus amount produced by cell cultures 3-5 days old were similar.
Inactivated vaccines prepared with DEAE-dextran or oil as an adjuvant induced good grptection for rabbit and good immunity for pigs. The average SN titers of pigs 4-6 weeks after second vaccination with oily vaccine were 1 :95, with DEAE-dextran vaccine, 1 :45. For rabbit, the 50% protection dose of DEAE-dextran vaccine was over 1: 18, of oily vaccine, 1: 1 I. They were free from side effects. It is suggested that the method is a potent approach to a large scale production of concentrated inactivated pseudorabies tissue culture vaccines.
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