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Reporton.
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AHRI report No.44
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Topic
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Establishment of Infectious Clone of Porcine Circovirus Type 2
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Department
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Animal Health Research Institute, Council of Agriculture, Executive Yuan
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Author
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C. Wang*, J. Y. Chiu, T. S. Huang, F. Lee, M. H. Jong, W. M. Chang
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Summary
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Porcine circovirus type 2 (PCV2) is associated with post-weaning multisystemic wasting syndrome (PMWS). PCV2 is an important infectious disease in swine and occasionally co-infects hosts with other pathogens. PCV2 is difficult to isolate from field samples, and is maintained at low titers in vitro. In this study, we have constructed the infectious clone of PCV2 to generate stable virus. The infectious clones were transfected into PK-15 cell line to generate new viral particles. The highest titer of newly generated viral particles was 105.5 TCID50/mL after a sixfold passage. In addition, there was no statistically significant differences between virus titer and infectious clones of various transfect dosages.
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Keyword
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porcine circovirus, infectious clone, transfect
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