| Summary |
In order to establish the diagnostic nucleic acid probe for detecting to egg drop syndrome virus ( EDSV) DNA, the genome ofEDSV CO strain isolated from chicken oviduct was cloned in pB!ueScript II KS ( + / -) vector. Eight DNA fragments in EDSV were cloned, but terminal genomic DNA were not. A specific digoxigenin ( DIG ) -labeled probe was prepared from a 1.5 kilobase-pair-cloned fragment. Six EDSV Taiwan isolates were detected by the nucleic acid probe method, but one avian adenovirus ( NO.9 strain) was not. The 100 pg volume ofEDSV DNA and infected duck embryonated fibroblast ( DEF ) cells were surveyed by southern blotting and DNA hybridization. The established DNA probe technique of this study can be used as a detective tool for infection of EDSV.
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