| Reportno. |
AHRI report No.41
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| Topic |
Determination of diethylstilbestrol in feed by high performance liquid chromatography
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| Department |
Animal Drugs Inspection Branch, Animal Health Research Institute Council of Agriculture, Executive Yuan
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| Author |
Chen SF , Liu CH* , Kuo MY, Su JF |
| Summary |
High performance liquid chromatography equipped with photodiode array detector (HPLC/PDA) was used to measure the quantity of diethylstilbestrol in feed. To prevent the false positive reaction caused by low concentration of diethylstilbestrol in feed, high performance liquid chromatography equipped with mass spectrophotometer (HPLC/MS) was further used as a confirmation test. The samples were prepared by stirring with methanol, followed by a centrifugation step and then an evaporation step. The residual is then dissolved with 70% methanol and the aqueous solution is cleaned by the assay of solid-phase extraction (SPE) using Oasis®HLB to remove the matrix interference, and the eluate was obtained and analyzed by HPLC/PDC or HPLC/MS. The result showed that the average recover rates of diethylstilbestrol in the spiked samples were during the range of 80.8 - 107.5%. The Limit of Detection (LOD) of PDA was 0.03 ppm and the LOD was 0.005 ppm when the MS was used.
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| Keyword |
High Performance Liquid Chromatography, Diethylstilbestrol, Feed
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