Hot Search

Update Date: [2022-09-28]

Publication Development of Tissue Culture Hog Cholera Vaccine


AHRI report No.24


Development of Tissue Culture Hog Cholera Vaccine


Taiwan Provincial Research Institute for Animal Health.

Author M.  H. Jong, C. C. Huang, I. P. Chan, T.H. Liu,C.W. Chi, T.F. Chiou, J. T. Lee

Pigs with 8 wPeks of age were vaccinated with live vaccine of LPC-China strain intramuscularly (IM) or intranasally (IN). All pigs vaccinated IN were sick and dead, and all pigs vaccinaged IM survived when they were challenged with a virulent strain 10 days after vaccination. Results implied that LPC-China strain could not replicate so well at lower temperature condition. 

rimary and established cell lines originated from pig kidney and testicle were used for serial passage of LPC-China strain virus. Results showed that a titer of 106.5 TCID50 /ml and 107-5 Rm50/ml could be reached when the tirus was prop-agated in PK-CL cell line. The virus, however, replicated poorly in primary swine kidney, testicle cell cultures and in ESK, ST-CL established cell lines. The virus replicated better at 37°C. 

Doses of 1/100, 1 / 1,000 and I/ 5,000 of the virus which has been serial passaged 6 times in PK-CL cell line, was inoculated IM in 8-week old pigs with low neutralizing antbody. All vaccinated pigs survived with a febrile reaction (over 41 °c) when they were chaJlenged 1 0 days after vaccination; on the other hand, all unvaccinated control pigs were killed afgter challenge. 


Hog Cholera Vaccine; LPC; intramuscularly


::: SiteMap - Switch ▲