| Summary |
Premixes were pretreated with a little volume of dimethylsulfoxide, then absolute ethanol was added to volume. After mixing and filtration, the filtrate was injected into a high performance liquid chromatography (HPLC) which was equipped with a Beckman Octyl 5 µ 4.6 x 250 mm column. Changing methanol concentration in mobile phase to 100 % 、98 % and 95 %, the retention times ofvitamine A' D3 and E could be changed and get good resolution if interferring peaks appeared. Using changeable UV wavelength ( 0 -5' 325 nm ， 5 -6' 265 nm, 6 -10' 284 nm ), the sensitivities of Vit. A and D3 in premixes could increase than fixed wavelength 280 nm, but Vit. E wasn't. By using eletrical chemical detection, Merck RP-18 5 µ 4.6 x 250 mm column, 0.1 M LiCIO4 Methanol : H2O = 99 : 1 mobile phase, alkaline saponified Vit. A 、 D3 standards could also extremely increase sensitivity, but two peaks were too does and vit. E retended too long.
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| Keyword |
High Performance Liquid Chromatography, Electric Chemical Detector Premix, Vitamin A , D3 , E.
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