1

Speaker(s)：

Chun Wang

Topic

Dual-infection of porcine circovirus type 2 and torque teno sus virus in Taiwan

Abstract

Porcine circovirus type 2 (PCV2) and torque teno sus virus (TTSuV) are the major emerging viral pathogens in Taiwanese pig farms. PCV2 is classified into two major genotypes: PCV2a and PCV2b. TTSuV can be divided into two distinct species, TTSuV1 and TTSuV2. In this study, 811 samples were collected from Taiwanese pig farms between 2001 and 2011. Among 811 samples, 140 (17.2%) samples were PCV2a positive, 573 (70.6%) samples were PCV2b positive, and 41 (5.1%) samples were positive for both PCV2a and PCV2b. The prevalence of TTSuV1, TTSuV2, and co-infection of TTSuV1 and TTSuV2 was 9.4%, 7.5%, and 79.5%, respectively. In addition, co-infection of PCV2b and TTSuV1/TTSuV2 is 56.5% (458/811). Our results suggest that PCV2 and TTSuV infections have become common in Taiwanese pig farms, respectively, and co-infection of PCV2b and TTSuV1/TTSuV2 also present high prevalence.

2

Speaker(s)：

Tsu-Han Chen

Topic

Standard diagnostic test of vesicular stomatitis

Abstract

There are three vesicular diseases of pigs which are difficult to differentiate clinically: vesicular stomatitis, foot-and-mouth disease, and swine vesicular disease. However, the envelope glycoprotein (GP) and nucleocapsid protein (N) of vesicular stomatitis virus (VSV) serotypes of New Jersey and Indiana can be used as a differentiated tool based on these proteins possessing a characteristic of high immunogenicity of the antigenic domain on their recombinant proteins. The study aimed to develop both competitive and indirect ELISAs for the detection of antibodies against VSV according to the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals published by OIE in 2012. Overall, both competitive and indirect ELISAs for antibody detections had been established based on experimental elements, such as convenience, stability, sensitivity, specificity and safety, etc. After testing the VSV antibodies in both infected bovine and horse sera, the analysis sensitivity and specificity had reached 10^-3.0 fold and up to 90.0%, respectively. The results indicated that the ELISAs can be used to detect anti-VSV antibody in the serum samples and do not cross-react with anti-FMDV antibody, so that would be used as a serological differential tool for vesicular diseases.

3

Speaker(s)：

Ming-Chung Deng

Topic

Case Report: Porcine Epidemic Diarrhea

Abstract

In January 2014, the suckling piglets exhibited severe diarrhea and death in pig farms in Pingtung and Yunlin County, Taiwan. The specimens were submitted to Animal Health Research Institute on January 21. It’s very difficult to differentiate transmissible gastroenteritis (TGE), porcine epidemic diarrhea (PED) and porcine rotavirus infection by clinical syndromes. According to virus isolation and RT-PCR, the preliminary diagnosis was PED infection. The PED was confirmed by transmission electron microscope (TEM) examination and gene sequencing. Furthermore, the partial M gene of isolated PEDV was compared with other PEDV sequence in Genbank. The isolated PEDV was 100% identity with US strain isolated in 2013, and 98.1% identity with classical strain CV-777. Until now, the outbreak of PED has gradually slowed down. The positive rate of PEDV was 75.61% (31/41), including 31 pig farms distributed in 9 counties.