Abstract
The aim of this study was to generate recombinant proteins from prokaryotic cell expression system for the differential detection of antibodies to FMD virus by multiplex Luminex (multi-analyte profiling; xMAP) assay. After optimization of the Luminex assay, it detected antibodies to both structural protein (SP) and non-structure protein (NSP) of FMD virus in a single serum sample. To detect SP antibodies in sera of infected pigs, naive pigs, and vaccinated pigs, diagnostic sensitivity (DSn) and diagnostic specificity (DSp) of the assay were 81.4-96.7% and 93.0-96.5%, respectively. To detect NSP antibodies, the DSn was 90% and DSp ranged 95.3-99.1%. In detecting SP and NSP antibodies in field pigs, the results demonstrated that xMAP had no relationship with virus neutralization test. The results indicated that the Luminex assay has potential in detecting antibodies to FMDV SP-VP1 and NSP-3ABC and in distinguishing FMDV-infected pigs from those infected with SVDV.
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Abstract
A specific CAE control programme adapted to clean house in one goat rearing flock. Determination of CAE disease prevalence should be the initial action, gradual culling of seropositive animals by ELISA and PCR diagnosis methods to reduce seroprevalance progressively every 3 month. Supplemented with health monitoring included goat pox, chlamydophila, Q fever and other diseases ensure that the animal health status. Under quarter testing head numbers were 132, 64, 149 and 101, respectively. And CAE ELISA antibody-positive rate were14.39%, 34.38%, 26.85% and 3.96%, respectively. CAE PCR Antigen positive rate of 1th quarter was 3.03%, 4th quarter was 0.99% and the rest were zero. Q fever ELISA antibody positive rate were 3, 39.39% and 12.87%. Q fever PCR positive rate in the 2nd quarter was 12.28%, remaining free. Bluetongue antibodies, chlamydophila PCR and goat pox PCR were negative. CAE Confirmed positive goats must be removing, Q fever PCR positive were eliminated also.
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