Monitoring of serum antibodies for the detection important swine viral pathogens in Taiwan
Chun Wang
Abstract
Classical swine fever virus (CSFV) and porcine reproductive and respiratory syndrome virus (PRRSV) are important viral pathogens in pigs. As specified in the World Organisation for Animal Health (OIE) Manual of Diagnostic Tests and Vaccines for Terrestrial Animal, serological diagnosis of CSFV and PRRSV in pigscan be performed using several methods, including enzyme linked immunosorbent assays (ELISA), indirect immunofluorescense assays (IFA), immunoperoxidase monolayer assays (IPMA), and serum neutralizing antibody assays (SNT). Since the neutralizing antibody detection method has many advantages such as ease of operation and low cost, we currently apply SNT for routineepidemiological surveillance for CSFV and PRRSV.
Proficiency Testing Scheme of NSP Antibody ELISADiagnostic Devices for Foot-and-Mouth Disease Virus
Tsu-Han Chen
Abstract
A domestic research team has jointly developed a highly accurate and sensitivein-vitro diagnostic kit (IVD) using a blocking enzyme-linked immunosorbent assay
(ELISA) and is commercially available as the Sentinel® FMDV NSP Ab ELISA kit.This kit is mainly to be used for the detection of non-structural protein (NSP)
antibodies of anti-foot-and-mouth disease virus (FMDV) which can differentiatevaccinated sera from infected sera. After evaluating this ELISA diagnostic kit using pig, cattle, and goat sera, we report diagnostic sensitivities and specificities as high as 95% or more. In recent years, the inter-laboratory validation of the proficiency testing scheme (PTS) has demonstrated high agreement and reproducibility of the results obtained between the Sentinel NSP ELISA, PrioCHECK, and UBI FMD-NS ELISA kits. Our participation in the PTS of the United Kingdom Foot-and-Mouth Disease reference laboratory in 2016 produced similarly satisfactory results with respect to bovine and porcine serum samples tested with the Sentinel® FMDV NSP Ab ELISA kit. Furthermore, the kit can specifically detect antibodies for the NSP of O/TW/97 and O/TW/99 FMDV serotypes as well as other serotypes in swine and bovine sera.We did not observe cross-reactions of the antibodies to swine vesicular disease and vesicular stomatitis viruses which further highlights this assay’s specificity.
The wild bird Avian Influenza Diagnosis and Surveillance Program at the South Korean Animal and Plant Quarantine Agency
Wan-Chen Li
Abstract
South Korea is often the first country to encounter a seasonal epidemic of the highly pathogenic avian influenza virus in East Asia. The notification of thes outbreaks serves to alert neighboring countries and the rest of the world. These outbreaks have often heavily impacted the poultry industry, and there are also many cases of concomitant avian influenza infections in wild birds. Among other data and analyses collected during these outbreaks, phylogenetic analyses of South Korean avian influenza viruses are an essential component to determining their evolution and predicting future outbreaks. Thus, we visited the Poultry Avian Influenza Research and Diagnostic Division of the Animal and Plant Quarantine Agency (APQA) in South Korea. The main content of the visit focused on the monitoring program for wild bird avian influenza in South Korea, which included assessing the cooperative and operative procedures between the APQA and other responsible organizations. In addition to disease monitoring, the APQP has employed from 2013 onwards, a novel global positioning system (GPS) tracker systems to record the flight routes of common migratory birds. The results have been applied to the analysis of wild bird transmission of avian influenza, and some of this information is disseminated to the public.