1

Speaker(s)：

Yu-Liang Huang

Topic

DPorcine reproductive and respiratory syndrome virus diagnostics

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) causes reproductive disorders in hog breeding herds and the respiratory dyspnea in piglets. Since the virus was detected in Taiwan in 1991 by virus isolation and RT-PCR, PRRSV has become widespread in Taiwanese farms and incurred serious economic losses. The methods for PRRSV detection commonly include virus isolation, molecular markers, and immunohistochemistry. Both virus isolation and molecular methods are generally used in the PRRS diagnosis at the AHRI. In 2016, a total of 18 farms tested PRRSV positive in the 27 PRRSV-suspected farms using molecular detection methods and 8 PRRSV strains were isolated. These PRRSV cases were all classified into the North America type by sequence analysis.

2

Speaker(s)：

Chien Tu

Topic

Report on the 15th Meeting of Asia Reginal Group on Aquatic Animal Health

Abstract

The 15th Meeting of Asia Reginal Group on Aquatic Anima1 Hea1th was he1d from November 21 to 23 in Bangkok, Thai1and. The members consist of countries from Association of Southeast Asian Nations (ASEAN) and China. The main sessions for discussion are food safety and pub1ic health for aquatic products, contro1 of new1y emerging aquatic diseases and survei11ance of drug resistance. Advisory group determined that pub1ic health caused by drug resistance of aquatic products, the accreditation for culture process and pre-examination for aquatic products, and epizootio1ogy,diagnosis and contro1 for emerging diseases wi11 be set up as action p1ans 2017.

3

Speaker(s)：

Nan-Ling Kuan

Topic

Characteristics of Pasteurella multocida isolated from goats and cattle

Abstract

From 2012 to 2016, 107 clinical cases in goats and 95 cases in cattle were submitted to the microbiology laboratory for bacterial isolation, and of which, 55.1% (59/107) in goats and 13.7% (13/95) in cattle were suspected of respiratory tract infection. Pasteurella multocida had the highest rate of isolation in cases of respiratory infection. In recent years, there have been only a few studies and investigations regarding this topic in Taiwan. The impact of Pasteurella multocida infections on ruminants might thus be underestimated. In this study, 27 isolates of Pasteurella multocida from goats and cattle were included for characterization. The predominant capsular type of Pasteurella multocida was type D, and the secondary was type A. The result of multilocus sequence typing (MLST) analysis revealed that the Pasteurella multocida isolates belonged to three new sequence types (ST). All isolates were susceptible to the antibiotics, amoxicillin/clavulanic acid, ceftiofur, florfenicol, and enrofloxacin.

4

Speaker(s)：

Ai-Ping Hsu

Topic

Use of Reverse Genetics Techniques for Development of Newcastle Disease Live Vaccine

and Analysis of Virulence Factor

Abstract

Newcastle Disease (ND) is a highly contagious avian disease and causes serious economic losses in the poultry industry annually. Thus, the implementation of ND vaccines is necessary for every poultry farm worldwide. The Animal Health Research Institute (AHRI) has successfully developed an ND live vaccine of genotype VII based on serially artificial attenuation from a wild type virus. Moreover, with reverse genetics techniques, the antigenic fragment of IBV (Infectious Bronchitis Virus) has been inserted into the above Newcastle Disease Virus (NDV) backbone for development of a bivalent vector vaccine, and the satisfactory results of efficacy and safety evaluation for NDV have been proved. For future patent application of this NDV (and its vector platform), reverse genetics techniques was also applied to recover recombinant NDVs to clarify the critical mutations leading to attenuation. It was discovered that seven out of 45 points mutation have been verified and determined as the key mutations. In addition, the discussions and learnings of NDV vector platform during the visit in Laboratory of Zoonotic Diseases of Gifu University, Japan will be presented in this seminar as well.