The personnel quota of the Accounting Services in the ADIB, AHRI was reduced during the government's personnel streamlining and organizational reengineering. At first, its accounting operation had been planned to be subsumed under the headquarters' accounting office. However, after careful consideration of the branch's healthy revenue and efficient business practices, it was decided not to make any proposed change, which would have created massively extra load of work for the head office anyway. Under the headquarters' directive and coordination, the branch office had to recruit someone among its certified staff to be in charge of the accounting business so as to ensure the continuity of the branch office's daily operation. In 2010, I was appointed to be responsible for the accounting business of the branch office, concurrent to my other job as a researcher at the AHRI.
Since the beginning of my tenure, the branch has delivered some excellent funding implementation results. The projects of animal health research development between 2018-2022 showed 99.92% of budget implementation efficiency, while in the general administrative affairs, the budget implementation efficiency reached a level of 99.5% for the same period. Furthermore, the execution rate of annual registration or processing fees was 100% and the miscellaneous incomes were also excellent between 2018-2022. In short, the generated revenues in this period exceeded our annual budgets. These results would not have been possible without the contribution provided by the high-quality inspection services delivered by our hard-working staff.
I have learned so much about the importance and constraints of abiding to rules and regulations civil servants have be faced with in their daily work since I took over this job, having experienced various legal challenges and internal control first hand. . During this brief introduction to some concepts of internal control and due diligent operation, I have shared with you from a vet’s perspective some key issues you need to keep in mind for your work. I wish my talk will be great for you.
Optimization and Validation of Real-time PCR for Detection of Decapod Iridescent Virus 1 MCP Gene
Decapod iridescent virus 1 (DIV1) is an emerging viral disease of crustaceans and infection of DIV1 causes mass mortality in farmed shrimp, prawns, and crayfish. In 2020, the Network of Asia-Pacific Aquaculture Centres (NACA) recommended and documented the diagnosis methods of levels I to III for this disease in the disease card. In 2021, the method were included in the World Organisation for Animal Health (WOAH) list of diseases. This study aimed to optimize and validate the real-time quantitative PCR (QPCR) method for the DIV1 major capsid protein (MCP) gene published by Qiu et al. in 2020 to establish a locally standardized process and assay performance range. According to the WOAH Manual about the validation procedures for infectious disease detection methods, the nucleic acid extraction solution of healthy white shrimp was used as the matrix for serial dilution of standard positive plasmid samples to optimize and evaluate the performance characteristics of this QPCR analysis. The results showed that the DNA template volume of the best test result was 4 µL. Its standard curve showed a high linear correlation within the range of 4.6 × 102–4.6 × 109 DNA copies/reaction, and the correlation coefficient R2 value was 0.996; the regression equation was Ct = -3.474·log (DIV1 DNA copies) + 42.023. The analytical specificity (ASp) and analytical sensitivity (ASe) of this QPCR assay were further analyzed to be 100% (CI 95%, 94.5%, 100.0%) and 92% (CI 95%, 82.6%, 97.3%), respectively, while the 100% limit of detection (LOD) was 4.6 × 102 copies/reaction. This QPCR has demonstrated good phase 1 analytical performance for detecting DIV1 and will facilitate future ongoing phase 2 diagnostic assay validation procedures.
Lumpy Skin Disease Virus Workshop at Dubai, UAE
One of the colleagues of the Animal Health Research Institute was invited to participate the Lumpy skin Disease Virus Workshop held at Dubai, United Arab Emirates on November 16 to 18, 2022. The Workshop was by The Pirbright Institute, Global Alliance for Livestock Veterinary Medicines, and Bill & Melinda Gates Foundation and aimed to bring key stakeholders together, to provide a forum for the latest research on this virus, to share best practice for the prevention and control, and to promote a capripoxvirus research community. Lumpy skin disease virus is spreading consistently around Asian countries. Culling infected animals alone cannot control this disease effectively. In addition to stamping-out, massive vaccination with quality live attenuated vaccine, movement control of livestock, vector control, and awareness and early detection are essential. Research gaps on this disease may include the methods for differentiation of infection from vaccination, correlation of immunity and protection, evaluation of immunity, strain differentiation, and assessment of new-technology vaccine candidate.