Functional Overview of the Technical Service Office
S.T. Kuo
Abstract
The functions provided by the Technical Service Office are extremely complex. In general, its current load can be categorized as: (1) reception and processing of documents/emails ; (2) management of the library as well as editing and publication of technical reports ; (3) management and application of research and development results; (4) arrangement of veterinary continuing education and training, conferences, meetings, and the reception of various domestic and foreign visitors; and (5) management of general affairs and other occasional missions which cannot be categorized as the responsibilities of other divisions of this institute. In today’s talk, we will give a brief introduction to the principle of technical appraise for research outcomes.
Establishment and application of materials formonitoring of importantswine viral pathogensin Taiwan
ChunWang
Abstract
Classical swine fever virus (CSFV) and porcine reproductiveand respiratory syndrome virus (PRRSV) are the import swine viral disease in Taiwan. However, the cell line,which widely used for CSFV and PRRSV, are not efficient and heterogeneous in terms of permissivity to viral infection. In order to acquire cell lines that can reliably produce CSFV and PRRSV in high titers, parent cells will be re-cloning by limiting dilution for CSFV and PRRSV propagation, respectively. In addition, this project intends to establish the necessary materials for the detection of antibodies of CSFV and PRRSV in pigs and to collect the serum of different ages of swine in domestic pig farms to analyze the positive course of serum and the time course of antibody production in pigs to provide necessary information for epidemic prevention, and improve animal epidemic prevention and ensure agricultural safety.Firstly, to acquire a homogeneous porcine kidney cell line that can reliably produce CSFV-LPC in high titers, cell clones that show high permissive phenotype to CSFV-LPC infection were derived from heterogeneous PK15 parent cells by limiting dilution and cell cloning. Maximum virus titers in PK15 newly generator cells was 107 tissue culture infective dose 50 (TCID50)/ml.Secondly, high permissive African green monkey kidney cells (MARC-145) cell line was cloned by limiting dilution to propagate PRRSV. Maximum PRRSV viruses yield in MARC-145 was 108 TCID50/mL.