1

Speaker(s)：

Tsu-Han Chen

Topic

Development of a Multiplex Luminex for detecting of antibodies to foot-and-mouth disease virus

Abstract

The aim of this study was to generate recombinant proteins from prokaryotic cell expression system for the differential detection of antibodies to FMD virus by multiplex Luminex (multi-analyte profiling; xMAP) assay. After optimization of the Luminex assay, it detected antibodies to both structural protein (SP) and non-structure protein (NSP) of FMD virus in a single serum sample. To detect SP antibodies in sera of infected pigs, naive pigs, and vaccinated pigs, diagnostic sensitivity (DSn) and diagnostic specificity (DSp) of the assay were 81.4-96.7% and 93.0-96.5%, respectively. To detect NSP antibodies, the DSn was 90% and DSp ranged 95.3-99.1%. In detecting SP and NSP antibodies in field pigs, the results demonstrated that xMAP had no relationship with virus neutralization test. The results indicated that the Luminex assay has potential in detecting antibodies to FMDV SP-VP1 and NSP-3ABC and in distinguishing FMDV-infected pigs from those infected with SVDV.

2

Speaker(s)：

Lu-Jen Ting

Topic

Strategies for extension and eradication of caprine arthritis encephalitis in goat breeding farm

Abstract

A specific CAE control programme adapted to clean house in one goat rearing flock. Determination of CAE disease prevalence should be the initial action, gradual culling of seropositive animals by ELISA and PCR diagnosis methods to reduce seroprevalance progressively every 3 month. Supplemented with health monitoring included goat pox, chlamydophila, Q fever and other diseases ensure that the animal health status. Under quarter testing head numbers were 132, 64, 149 and 101, respectively. And CAE ELISA antibody-positive rate were14.39%, 34.38%, 26.85% and 3.96%, respectively.  CAE PCR Antigen positive rate of 1th quarter was 3.03%, 4th quarter was 0.99% and the rest were zero. Q fever ELISA antibody positive rate were 3, 39.39% and 12.87%. Q fever PCR positive rate in the 2nd quarter was 12.28%, remaining free. Bluetongue antibodies, chlamydophila PCR and goat pox PCR were negative. CAE Confirmed positive goats must be removing, Q fever PCR positive were eliminated also.

3

Speaker(s)：

Shu-Chia Hu

Topic

Comparison of the effects on maternally-derived antibodies level of pig from different FMD vaccination program immunized sows

Abstract

The FMD vaccination regulations of pig is vaccinated once dose at 12~14 weeks of age and followed by a regular booster vaccination six months later. Depend on the vaccination regulation, different vaccination program of sows are used on the farms, and the most common program is revaccination during open-days. Few reports are available on the uniformity and duration of maternally-derived antibodies level in offsprings born from using different vaccination program in sows. The aim of this study was to compare the deviation and duration of maternally-derived antibody level in pigs from two different vaccination groups of sows. The results showed geometric Ab and antibody distribution of the offspring at 12 weeks of age of different groups without significant difference. However, the result is needed to prove in the future due to the selection bias and small sample size of sows.

4

Speaker(s)：

Jen-Chieh Chang, Yang-Chang Tu

Topic

The study of avian influenza diagnoses and researches at Hokkaido University in Japan

Abstract

AHRI dispatched two staffs to study the diagnoses and researches of avian influenza (AI) including reverse transcription polymerase chain reaction (RT-PCR), the analysis of nucleic acid sequencing, intravenous pathogenicity index (IVPI), chicken embryo inoculation, hemagglutination assay (HA), Hemagglutination inhibition test (HI), plaque assay and real-time polymerase chain reaction (RT-PCR). We also discussed that we try to participate in proficiency panels from AI OIE Reference Laboratory and cooperate in the future. Completion of this training greatly strengthens our understanding of AI diagnoses and researches in Japan. That would be helpful to our researches in the future and the strategy of AI prevention. It also promotes the bilateral cooperation between Taiwan and Japan.