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Seminar 930

Date:2022-05-03 Update:2022-05-03

Antimicrobial Resistance in Escherichia coli from Diseased Animals

Nan-Ling Kuan


Extended-spectrum β-lactamases (ESBLs) are a rapidly evolving group of β-lactamases that shares the ability to hydrolyze third-generation cephalosporins, penicillins, and aztreonam. Since 2000, ESBL-producing Escherichia coli (ESBL-EC) infections have increased worldwide in human, food animals, and companion animals. In this study, 569 isolates of E. coli from diseased animal were obtained from 2013 to 2020. Sixty-seven isolates ESBL-ECs out of 569 isolates were confirmed by phenotyping and genotyping, which were mainly presented as bla genes, such as blaCTX-M (77.6%), blaTEM (14.9%), and blaSHV (1.5%), and some isolates were carried bla genes from two groups. The dominant bla genes variant was blaCTX-M-55, followed by blaCTX-M-14. All ESBL-ECs were resistant to tetracycline, predominantly ceftiofur and SXT (92.5-97%), followed by gentamycin (64.2%), enrofloxacin (53.7%), and amoxicillin (32.8%). All isolates remained susceptible to imipenem.



Current epidemic status of bovine tuberculosis in Taiwan cattle

Chen-Shen Huang


Bovine tuberculosis (bTB) is an important zoonotic disease caused by Mycobacterium bovis. Cattle are considered to be the main hosts of M. bovis. Meanwhile, most mammals can be infected. Depending on the infection routes, characteristic nodular lesions can be observed in lymph nodes of the head and chest, or in abdominal organs. The intradermal tuberculin test (ITT) is the standard method for testing bovine tuberculosis, in particular, for international trade. It is also the standard method for bovine tuberculosis detection in Taiwan. We analyzed the bTB positive cases in Taiwan from 2019 to 2021. Since the second half of 2020, many cases have been found in slaughterhouses, and the number of positive farms increased. The M. bovis isolates were genotyped by two genotyping methods commonly used in Mycobacterium tuberculosis complex, spoligotyping and MIRU/VNTR (three loci, ETR-A, ETR-B, and Qub11b), for molecular epidemiological analysis. A total of 12 genotypes were obtained in 2021, the most in history.

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