Abstract
The study hoped to develop an ELISA for the detection of duck serum antibodies against Riemerella anatipestifer antigens. The serum samples including:serum from RA-free duck field、artificial infected ducks,and from ducks vaccinated with bacterins.
It has showed that serum from RA-free duck field had very low absorbance at 405nm using ELISA,on the contrary, artificial infected serum had pretty high one.
Serum from immunized with trivalent bacterins (serotype 1、2 and 6 combined) appeared significantly deviation compared to RA-free serum measured with ELISA.Three serotypes antibodies kept rising after vaccination, antibodies of serotype 1 stayed high level at 6th to 14th week; antibodies of serotype 2 also reached high at 8th to 11th week; antibodies of serotype 6 went up suddenly since at 5th week and persisted on high plateau at least at 15th week.
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