1

Speaker(s)：

Yu, Chao fang

Topic

Establishment of an ELISA for the Detection of Duck Serum Antibodies against Riemerella anatipestifer Antigens

Abstract

The study hoped to develop an ELISA for the detection of duck serum antibodies against Riemerella anatipestifer antigens. The serum samples including：serum from RA-free duck field、artificial infected ducks,and from ducks vaccinated with bacterins.

It has showed that serum from RA-free duck field had very low absorbance at 405nm using ELISA,on the contrary, artificial infected serum had pretty high one.

Serum from immunized with trivalent bacterins (serotype 1、2 and 6 combined) appeared significantly deviation compared to RA-free serum measured with ELISA.Three serotypes antibodies kept rising after vaccination, antibodies of serotype 1 stayed high level at 6th to 14th week; antibodies of serotype 2 also reached high at 8th to 11th week; antibodies of serotype 6 went up suddenly since at 5th week and persisted on high plateau at least at 15th week.

2

Speaker(s)：

Ching-hui Kuo

Topic

Establishment of Genotyping for Salmonella enterica serovar Choleraesuis Using Pulsed-Field Gel Electrophoresis (PFGE)

Abstract

Salmonella enterica serovar Choleraesuis is an important zoonotic pathogen, which usually causes septicemia, pneumonia and diarrhea in nursery or finisher pigs and systemic infection in humans. An epidemiological analysis was performed to determine the genotypes of 43 Salmonella enterica serovar Choleraesuis (6,7: c:1,5) isolates identified by biochemical characteristic and serological approaches. Genotyping was done by restriction enzyme of whole genome（Xba I）and pulsed-field gel electrophoresis (PFGE). Seven different genotypes were identified when the PFGE patterns were analyzed by GeneProfiler® and Treecon ®, the major group contained thirty five strains （81.3％）, the second and third group had two strains each （4.6％）, the rest groups were independent each （2.3％）. This study shows that PFGE is a highly stable and reproducible technique which can be used as a potent discriminatory method in epidemiological study.