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1
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Speaker(s)：
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Ming-Shiuh Lee
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Topic
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The identification of frequent variations in the fusion protein of canine
distemper virus
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Abstract
Canine distemper (CD) is a highly contagious disease with a worldwide distribution. Genetic diversity in genes encoding the haemagglutinin (H) and fusion (F) virus envelope proteins have been implicated in the increasing incidence of CD. Unlike the H gene, little is known about the genetic variability of the F gene in this virus. In the present study sequence analysis of the complete coding region of the F protein from CD virus isolates from Taiwan were carried out. Phylogenetic analysis demonstrated that the majority of isolates were similar to those found in neighbouring China and Japan, but were genetically distinct from vaccine strains. Remarkable variations were found scattered throughout the pre-peptide region (residues 1-135). The sequence identity of this region between locally sourced strains and between these strains and vaccine strains was 89% and 64 to 67%, respectively. Analysis suggested a novel strain of distant genetic lineage was present in dogs in the geographically isolated city of Hualien.
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2
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Speaker(s)：
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S.T. Kuo
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Topic
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The introduction to the morphology of significant pathogens in cases using EM
from 2000 to 2009
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Abstract
In recent years, the electron microscope performance and accessory devices were promoted and the observation of viral structure and the estimation of viral size become simple and direct. From 2000 to 2009, we found out many important viruses, including bovine food and mouth disease virus(2000), porcine Teschovirus (2000); porcine circovirus (2001); piscine viral nervous necrosis virus (2002), porcine Sagiyama virus (2002), koi herpesvirus (2002); bluetongue virus (2003), Holiotis sieboldii herpesvirus (2004); contagious pustular dermatitis virus (2006) and sheep pox virus (2008).
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3
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Speaker(s)：
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Chao-Fang Yu, Chin-Cheng Huang
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Topic
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Detection of Sera Antibody by ELISA from Field Trial Waterfowl Immunized
with Trivalent Bacterin againstRiemerella anatipestiferInfection
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Abstract
This study followed waterfowl field trial about trivalent inactivated bacterin against Riemerella anatipestiferinfection last year, we detected sera antibody titers by ELISA from immunized and non-immunized groups subsequently. The results indicated that ducks and goose obtained high sera ELISA antibody titers after vaccination, and the high titers lasted till slaughter age. Sample/negative ratio were 1.4~12.8.
Additionally, the assay was shown to be reproducible both within and between-assay for high, medium and low levels of antibodies to RA. The degree of specificity of ELISA was demonstrated in antisera to duck hepatitis, fowl cholera and colibacillosis, gave no responses exceeding the positive threshold value (OD405nm=0.4) for the assay.
Tube agglutination test was also performed for detecting sera antibody of field trial (still in progress), and then compared with ELISA. However, the agreement for these two methods were 50~80%.
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4
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Speaker(s)：
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Ming-Chu Cheng
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Topic
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Avian Influenza surveillance in Wild Birds in Taiwan 2008
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Abstract
In 2008, a total of 4,265 specimens collected from the birds in Taiwan major wild bird habitat and wild bird rescue center and wild bird medical station were examined for avian influenza viruses. Twenty-six viruses were isolated (0.61% isolated rate) and subtyped as H2N9 (n = 1), H3N6 (n = 3), H3N8 (n = 5), H4N2 (n = 3), H4N6 (n = 4), H7N3 (n = 2), H7N7 (n =2), H10N1 (n =1), H10N7 (n = 2), H10N9 (n = 2) and H11N3 (n=1). Twenty-two of these total viruses were isolated from specimens of ducks and four of them from shorebirds. Four H7- subtype viruses were isolated in the wetlands of Tainan and Yilan during January and February. These viruses were identified as the nonpathogenic strains by the results IVPI test and the amino acid moti at cleavage site of hemagglutinin protein. In the results indicated that, in 2008, the risk of HPAI H5N1 virus carried by migratory bird to Taiwan is low, and no any avian influenza viruses be fund in the specimens of wounded, sick or dead birds.
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