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1
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Speaker(s)：
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Huang , Yu-Liang
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Topic
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The effect of LPC vaccination in PCV2-infected pigs
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Abstract
Porcine circovirus type 2 (PCV2) is infectious topost-weaning pigs of 25 to 120 days old andrelated to post-weaning multisystemic wasting syndrome (PMWS) that manifests in severe immunosuppression. Most PMWS cases are associated with PCV2 infection, but PCV2 infection dose not always cause PMWS. It has been known that PMWS is correlated with the enhancement of other factors in PCV2 infected pigs. Until now, Mycoplasma hyopneumoniae,porcine parvovirus (PPV), porcine reproductive and respiratory syndrome virus (PRRSV), vaccination or chemicalimmune-suppression and immune-modulation have been reported to be able to enhance development of PMWS from PCV2-infected pigs. In addition, the LPC vaccine is used to prevent classical swine fever in Taiwan and vaccination programs apply to pigs of 3- tp 6-weeks of age or 6- to 9-weeks of age. The PCV2-susceptible age for pigs is known to overlap withvaccination programs of the LPC vaccines. In the present study, the effect of LPC vaccination in the PCV2-infected pigs was evaluated by in vivoexperiments. We found that LPC vaccinations could improve diarrheal symptoms in the PCV2-infected pigs and led to decreases in feed conversion rate. The characteristics of WBC, PCV2 viral load and PCV2 antibodies in PCV2-infected pigs were not affected by LPC vaccination.
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2
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Speaker(s)：
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Chiang, Han
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Topic
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Developement of a Immunomagnetic Bead Diagnostic Reagent for Newcastle Disease
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Abstract
This study adopted affinity immunomagnetized antibody/antigen, co-immunoprecipitation, and genoamplification techniques so as to produce a Newcastle disease magnet bead diagnostic reagent for improving and enhancing diagnostic specificity and sensitivity. Since the major antigenicity regions are located in NP, HN and F genes, and with F gene’s variant nature, we constructed both HN-F and NP-HN fusion proteins as antigens that are conversely expressed as inclusion body forms by prokaryote expression systems and should be refolded to become soluble proteins. So far, we have conducted the expression, purification, stabilizing tests and preparation of HN-F protein for hybridoma. In the next study, we will set out to produce the immobilized magnetic beads on antibodies and analyze the interaction of immunomagnetized antibodies and antigens by applying the QSM (define this) method.
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3
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Speaker(s)：
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Hwang , Chyi-Sing
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Topic
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Construction of a Silkworm Challenge Laboratory
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Abstract
In order to improve the process of vaccine production, reduce costs, and increase vaccine efficacy, a silkworm production platform for E2 subunit vaccine of classical swine fever virus was developed. The platform utilizes a baculovirus called Bombyx mori nuclear poly- hedrosis virus (BmNPV) as a vector to infect silkworms to overexpress foreign proteins. In the eukaryotic silkworm expression system, the overexpressed protein can be modified by glycosylation and/or phosphorylation to form correct structures and they possess a high level of bioactivity. The BmNPV only infects silkworms and produces no biohazard if it is spread into the environment. Compared with the protein expression of fermentation tanks and tissue culture, utilizing silkworms as bioreactors to overexpress engineered protein is of low-cost and highly efficient. For large-scale production and commercialization of E2 subunit vaccine, the construction of a silkworm challenge laboratory was therefore necessary for us. In light of this, using the facilities and resources in AHRI , we renovated a room as a miniature model of a silkworm challenge laboratory in 2009. This laboratory was set up as a controlled environment at a constant temperature of 25+ 2℃, with constant humidity (60+ 5%)and air pressure (＞5Pa). According to the classes of air cleanliness levels in cleanrooms and clean zones, there are three classes of clean zones. Zone 1－ a class 100 cleanroom － never allows more than 100 particles (0.5 microns or larger) per cubic foot of air, and is designed to operate aseptically. Zone 2 and zone 3 are Class 10,000 and Class 100,000 cleanrooms, respectively. There are 2 air-conditioning systems and 1 supervisory control system in this laboratory. The laboratory floor is made of EPOXY with fiberglass reinforced plastics (FRP). These constructions were designed as a model for the small-scale production of silkworm protein expression systems. We will develop mechanical facilities to decrease manpower in the process of vaccine production which will also make expansion to large-scale production very smoothly.
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