Development of a Newcastle disease live vaccine of genotype VII and the establishment of a reverse genetics platform

Ai-Ping Hsu

Abstract

Newcastle Disease (ND) is a highly contagious avian disease and causes serious economic losses in the poultry industry annually. Thus, the implementation of ND vaccines is necessary for every poultry farm worldwide. The Animal Health Research Institute (AHRI) has successfully developed a ND live vaccine of genotype VII based on serial, artificial attenuation from a wild type virus. Moreover, to modify this vaccine strain, by establishing reverse genetics technique, the safety and antigenic properties were further enhanced. The results of efficacy, safety, pathogenicity, and virulence reversion were all satisfactory and complied to the assay standards for ND live vaccine in both European Union and Taiwan. In addition, the reverse genetics technique was also used to assess the virulence factor of the vaccine strain, the novel attenuation mechanism was discovered, and we thus accordingly applied for a patent this year. In this seminar the development process for the live vaccine, the achievements obtained, and its future application (the invention of multivalent vector vaccine platform) will be presented as well.

Participation at the2017 Global Foot Mouth Disease Research AllianceScientific Meeting, in Incheon, South Koreaandat theannualmeeting in Tokyo, Japan onthe collaborativeproject“Developing the Novel Method for Classical Swine Fever and High Pathogenic Porcine Reproductive and Respiratory SyndromeDiagnosis”

Ming Chung Deng

Abstract

The Global Foot and Mouth Disease Research Alliance(GFRA) wasstarted in 2003 by the Agricultural Research Service(ARS) of the US Department of Agriculture(USDA).The goal of the GFRA is to coordinate a global alliance of FMD research laboratoriesand institutes for sharing results and discoveries that enableus to successfully prevent, control and eradicate FMD. The 2017 scientific meeting of GFRA was held atIncheon, South Korea. Dr. Yeon-Liang Lin and Dr. Ming-Chung Deng participatedin this meeting and presented two posters entitled”Study on the vicissitude of antibodies induced by vaccinating a single dose high potency FMD vaccine in SPF and commercial pigs” and “Comparison of the efficacy among the O/Taiwan/98, O1/Campos and O1/Manisa strain FMD vaccines in the commercial swine herds“ For monitoring, disease control and diagnosis kit development ofclassical swine fever and highlypathogenic porcine reproductive and respiratory syndrome, our institute signed aMemorandum of Understanding (MOU) with the National Institute of Animal Health, in Japan on April 2014. The MOU expired on March 2018, and Dr Chia-Yi Chang was invited to presentour study entitled:”Development and validation of new diagnostic methods of highly pathogenic porcine reproductive and respiratory syndrome (PRRS) and classical swine fever (CSF)”. Furthermore, for research, disease control and diagnosis kit development on classical swine fever and African swine fever, we signed a mutual agreementon cooperation between our two institutes.