The quality surveillance of the post-market veterinary

Chen Yu-Lin

Abstract

The life cycle of veterinary drugs is lengthy, and the quality surveillance in the life cycle is very complicated. The quality surveillance of post-market veterinary drugs is one of the important part of the veterinary drugs quality management policies. Our project is based on the Veterinary Drugs Control Act and veterinary drugs inspection standard. We provide the post-market veterinary drugs ingredients, quality and quantity of quality inspection in collaboration with local authority’s policy of randomly sampling on the market. These inspection results will become part of the veterinary drugs risk assessment data. To ensure veterinary drug safety and promoting the development of veterinary drugs industry. In the analysis of the inspection results of the post-market veterinary general drugs in the recent years, more than 70% of sampling drugs was domestic products. The pass rate of inspected drugs was more than 95.9%. In addition, the results showed the main unqualified drug was Penicillin G.

Detection of Bovine Ephemeral Fever Virus Using Real-Time RT-PCR

Lu-Jen Ting

Abstract

The method developed in this study was based on the Taqman probe quantitative reverse transcription polymerase chain reaction, or qRT-PCR, of the Australian Animal Health Laboratory.The primers and probe sequences of our method were slightly modified to be suitable for in the detection of Taiwan bovine ephemeral fever virus (BEFV) strains. Tested on 6 strains isolated from 1984 to 2015, the detection limit was1 to 10 TCID50/mL, more sensitive to thenested PCR currently used in our laboratory.When applying the new method to detect the Taiwan’s new BEFV genotype, found in 2015, we found thattwo point mutations within the region of probe annealing, generated during the second passage of the virus,may negatively affect the qRT-PCR fluorescence reaction. Therefore, the probes and primers were redesigned, and three BEFV strains were tested. The detection limit was 100TCID50/mL, similar to mutant undetected probe and primers, but the fluorescence intensity increased by more than 1.2 times. The mutant virus strain can be also detected.To evaluate the sensitivity between the new qRT-PCR, nested PCR, and conventional RT-PCR, 27 BEFV-positive blood samples were tested.Our results demonstrated that the sensitivity of these methods were 100%, 96%, and 74%, respectively, supporting thattheqRT-PCR we developed is a sensitive tool for detecting BEFVs which have been present in Taiwan.