1

Speaker(s)：

Yu-Liang Huang

title

The effect of porcine circovirus type 2 on classical swine fever virus-induced proliferation of porcine peripheral blood mononuclear cells

Abstract

Porcine circovirus type 2 (PCV2) is infectious to post-weaning pigs between 25 and 120 days old and proves to down-regulate the immune response of swine. The PCV2-susceptible age of pigs is known to overlap with the vaccination programs of the LPC vaccines at 3- and 6-week of age or 6- and 9-week of age. In the present study, the effect of PCV2 on cell mediated immunity (CMI) was evaluated by classical swine fever virus (CSFV)-induced proliferation of peripheral blood mononuclear cells (PBMC). We found that in vitro CSFV-induced proliferation of PBMC could be induced with the stimulation of 0.1 MOI of ALD strain for at least 4 days. Moreover, the effect of PCV2 on CSFV-induced proliferation of PBMC was examined and found that PCV2 was able to significantly reduce the viability, CD25 expression, and CSFV-induced proliferation of PBMC (P < 0.05). These results also indicate that PCV2 was able to suppress the CSFV-induced proliferation of PBMC and could subsequently affect the ability of CMI against CSFV.

2

Speaker(s)：

Wei-Ming, Chang

title

Seroprevalence of cattle and goat melioidosis in Taiwan

Abstract

To investigate the seroprevalence of goat and cattle antibodies against Burkhoderia pseudomallei, both an enzyme-linked immunosorbent assay (ELISA) and a complement fixation test (CFT) were conducted. In this study, goat sera collected from various areas of Taiwan in different time, including 300 goat sera collected in 2008 from 4 counties located in southern and middle-western Taiwan, 650 goat sera collected in 2007 from 4 counties located in southern and southeastern Taiwan, and 634 cattle sera collected in 2007 from 4 counties located in southern and southeastern Taiwan, were tested. All tested sera, except 2 goat sera collected in 2007, showed negative by both ELISA and CFT. The 2 positive goat sera had CFT titer of 1:20 and 1:10, respectively. The overall seroprevalence of melioidosis for cattle and goats were 0 and 0.21 %.

3

Speaker(s)：

Sue-Min, Haung

title

Development of an immunochromatographic test kit for a rapid detection of iridoviruses infection in aquatic animals

Abstract

Iridovirus belongs to a family of icosahedral double-stranded DNA viruses and has a size of 120-300 nm. In the last decade, it was demonstrated that the virus could cause infection more than 30 fish species and the cumulative mortality of grouper fry could reach to 50-90% over 2 months. The family of Iridoviridae has been subdivided into five genera including Iridovirus, Chlorirdovirus, Ranavirus, Lymphocystivirus and Megalocystivirus. Among them, Ranavirus and Megalocystivirus can cause systemic diseases in infected animals and are associated with a high morbidity and mortality. Both of them have been found in Taiwan. To detect them, a rapid and simple immunochromatography (IC) strip test was developed by our lab for this study using mouse monoclonal antibody (against the major capsid protein) and rabbit polyclonal antibody for the specific detection of iridovirus. The lowest limit the fast detection strip reached is 104TCID50/mL. However, the IC strip test was designed specifically against iridovirus but not for the detection of the virus nervous necrosis and Lymphocystivirus. In our study, we were able to generate a specific MAb to detect both ranavirus and megalocytivirus. The MAb targets specifically the conservative region of the major capsid protein found in ranavirus and megalocytivirus. Most important, the strip test is easy to perform by untrained personnel at pond sites as results could be obtained within 15 min. and determined with naked eyes. Therefore, it is highly suitable as the front line monitoring infection. The strip test sensitivity of detection limits is 104TCID50 which can early determine the disease outbreak and reduce breeders’ economic losses.

4

Speaker(s)：

Kuo-Jung Tsai

title

The training report on pathological diagnosis and molecular immunological diagnosis techniques for zoonoses in CDC, USA

Abstract

The emerging and re-emerging zoonoses have drastically increased and widely expanded and thus become important global issues as a result of a globalized trade, climate change and the increased frequency for trade of animals and products of animal origin. In order to strengthen the diagnosis and surveillance techniques for major zoonotic diseases and better conduct the collaborative research programs between Taiwan and USA, two assistant researchers from the Animal Health Research Institute in Taiwan were dispatched to CDC in Atlanta, USA to learn about pathological diagnosis and molecular immunological diagnosis techniques for zoonoses during 16th to 24th of November in 2008. The training topics included immunohistochemistry (IHC), in situ hybridization (ISH), direct rapid immunohistochemical test (DRIT) and detection for pathogen nucleic acids from paraffin embedded tissues. It was aimed to apply above diagnosis techniques to zoonoses diagnosis and surveillance programs to help prevent the invasion of threatening zoonoses and their early screening.