The research of survival conditions of mammalian cells in specific pathogen-free chicken embryonated eggs
Ling-Chu Hung
Abstract
Chicken eggs and embryos that have not reached the 17th day of their development period are medically deemed to be unconscious. In the European Union, they do not require protocol approval by the Institutional Animal Care and Use Committee, nor do they need mammalian breeding facilities or ethical restrictions. In addition, the rich nutrient condition of eggs is suitable for the growth of poultry cells and chicken embryos. The purpose of this study was to develop the mammalian cell culturing technology by using specific pathogen-free (SPF) eggs. The evaluation of the survival conditions of mammalian cells in ovo was conducted. It was found that some mammalian cells could be implanted into the chorioallantoic membrane of SPF eggs between 7 and 10 days of incubation. The embryonic eggs could survive and the grafts were visible by magnification. After chorioallantoic membranes were collected, data showed some mammalian cells could survive on the chorioallantoic membrane of the embryonated chicken eggs by histological examination. Using of SPF chicken embryonated eggs for mammalian cell testing technology would reduce experimental costs. Further, it could be a reference for the experimental model in replacing or reducing mammals.
The establishment and analysis of a pathogen distribution survey model in poultry farms.
Yu-Hua Shih
Abstract
Livestock and poultry are an important part for food security. Some poultry farms lack biosecurity infrastructure and proper management protocols, resulting in a high risk of disease and low production efficiency of the raised poultry. Reducing production risks and stabilizing the quality of chick and egg supply are crucial.The poultry farm environment pathogen distribution survey utilizes real-time quantitative polymerase chain reaction to detect important avian viral diseases in the farm environment before and after disinfection. In breeder chicken farms, tests are conducted for Marek’s disease virus, infectious bursal disease virus, infectious bronchitis virus, and avian anemia virus. In breeder duck and goose farms, tests are conducted for Muscovy duck parvovirus, goose parvovirus, duck circovirus and goose circovirus.The results from 2023 showed the presence of Marek’s disease virus, infectious bronchitis virus, avian anemia virus, duck circovirus, and goose circovirus in the environment. After cleaning and disinfection, the number of bacteria and specific viruses in the breeder farms and hatchery environments was significantly reduced.This study also assessed the antibody titers of waterfowl circovirus vaccines in laying female Roman geese. The results showed that geese vaccinated with two doses of the vaccine had protective transitory antibodies in their 1-day-old goslings at the beginning, middle, and end of the production season, with proportions of 100%, 88%, and 75%, respectively. It is recommended to complete two doses of vaccination before the start of egg-laying.
Regional Workshop on Avian Disease Prevention and Control in Asia and the Pacific 2024
Li-Hsuan Chen
Abstract
The " Regional Workshop on Avian Disease Prevention and Control in Asia and the Pacific 2024" was held from August 27 to 29, 2024, in Seoul, South Korea. This seminar was organized by the World Organization for Animal Health (WOAH) as the regional laboratory expert network, encouraging members to share epidemic information. Its ultimate goal is to prevent the spread of avian influenza, protect the poultry value chain, and ensure public health and ecosystem safety, providing a sustainable framework for disease prevention and control for all members. Topics discussed at the workshop included recent developments in avian influenza and other avian diseases, national surveillance programs and control measures, as well as insights from 「One Health research」, aimed at supporting multisectoral partners to enhance response capabilities for avian influenza. As one of the attendee, the report for the seminar included information on the scale of Taiwan’s poultry industry, the current status of avian influenza, and surveillance plans. During the workshop, I engaged in exchanges on surveillance strategies and the latest technologies with various participants, yielding substantial insights and establishing ongoing communication with expert laboratories.