| Summary |
Premixes were added directly with mobile phase to a certain volume. After mixing and filtration, the filtrate was injected into a high performance liquid chromatography (HPLC) which was equipped with a BECKMAN OCTYL 5µ 4.6 X 250 mm column. The composition of mobile phase was the mixture of 5 mM PICB5 solution ( pH adjusted to 3.25 with acetic acid ) and Methanol in the ratio of 70 : 30 changing into 75 : 25. The peaks of the four vitamins were separated better as the ratio of 5 mM PICB5 solution was gradually increased in quantify. The detection sensitivity ofVit. B1 , B6 , and Niacin by using program changeable UV detector (0~ 3.8', 260 nm; 3.8~ 4.8', 290 nm; 4. 8~6.0', 246 nm 6.0~7.0', 266 nm) was higer than that by using fixed wavelength at 275 nm, while it was unchanged for Vit. B2.
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