The genotypes study of Mycobacterium bovis in 2014 Taiwan

CS Huang*, C Tu, HJ Tsai

Animal Health Research Institute, Council of Agriculture, Executive Yuan

Abstract

From October, 2013 to October, 2014, 31 Mycobacterium bovis samples were isolated from 44 intradermal tuberculin test (ITT) positive cattle (positive rate 40.9%, 18/44). In these cattle, the rate of nodule development and nodular animals with positive culture were 36.4% (16/44) and 87.5%(14/16), respectively. The spoligotypes of 31 M. bovis isolates were identified as SB0265 (61.3%) in 19 isolates, SB0140 (32.3%) in 10, and SB1040 (6.5%) in the case of two isolates. SB0265 was the most prevalent strain in Taiwan. SB0140 was found only in Chiayi and Hsinchu. SB0140 was isolated in Yunlin, Chiayi, and Pingtung. SB0140 disappeared from Pingtung for a 3 year period, but was uncovered in this province this year. The clusters of bovine TB positive herds appeared in 2 areas, the herds in A area were close to each other (<1km) and the herds in B area were at a distance of 5-8 km from one another. Both A and B groups have herds infected with both SB0265 and SB0140, indicating more than one infection source.

Keywords: Mycobacterium bovis；bovine tuberculosis；genotyping