Reporton.

AHRI report No.43

Topic

The antigenicity of extracellular products of Photobacterium damselae subsp. piscicida in cobia (Rachycentron canadum)

Department

Animal Health Research Institute, Council of Agriculture, Executive Yuan¹,

Tungkang Technology research center, Fisheries Research Institute, Council of Agriculture, Executive Yuan²

Author

Tu C*, Chiou RY, Huang SM, Chang WM and Chao PH

Summary

The purpose of this study is to screen the major antigenic protein moieties of the extracellular products ofPhotobacterium damselae subsp. piscicida in cobia for vaccine development. The antigenic proteins are verified through Western blot of the extracellular products reacted with the cobia antiserum against the bacteria. The collected antigenic bands were sent for mass spectrometry anaylysis and the peptide masses were compared with the protein sequences in GenBank. One 43 kDa protein was identified as a bacterial lipase and further research on its biological function will be done.

Keyword

antigenicity, cobia, photobacterium damselae subsp. piscicida,