Genotype of Porcine Rotavirus in Taiwan in 2014
YL Huang*, MC Deng, CY Chang, TS Huang, HJ Tsai
Animal Health Research Institute, Council of Agriculture
Abstract
Rotaviruses are important causative agents for swine diarrhea and are classified into 35 genotypes by the VP4 locus and 27 genotypes by the VP7 locus sequences. Vaccines use to protect swine induce little, if any cross protection between the many VP4 and VP7 genotypes, so genotype identity is crucial in responses to outbreaks . The aim of this study is to survey porcine Rotavirus in swine diarrhea outbreaks during 2014. A total of 86 farms with 242 fecal samples tested as positive for transmissible gastroenteritis virus, porcine epidemic diarrhea virus, and porcine Rotavirus by RT-PCR. The results showed that the predominant pathogen of swine diarrhea was porcine epidemic diarrhea virus. Porcine rotavirus was found only in 4 farms with 12 diarrhea samples and was classified as a G9P19 genotype by phylogenetic analysis of VP4 and VP7. The sequences of VP4 and VP7 in the Rotavirus isolated in this study were less than 90 % homologous than those found in strains from NCBI gene bank, including animal and human sources. These results suggested that the porcine Rotavirus strains discussed here are novel ones originating from Taiwan.
Key words: Rotavirus, diarrhea, viral protein 4 and 7