A BET-inactivated subunit vaccine against pseudcnbies virus was uepxed by treating a mixture of the PEG cc.ncenttatd virus fluid and infected cells with the detergent NP-40 when the volume of the treatl mixture was Irought up to 1/2 and 1/4 oftheoriginalvirus fiuidwithMEM. However, theefficacyof thevathnewas not sadsf&try.
Another detergent, Triton X-1CO, was used to teat the infecte,J cells, half ci tue mixture was then ultracenirifugated at 1 10,CXX) xg and the rest half was inactivated by EEl. Fresh MEM was a&kd into the surernant and the EEl inactivated mixture to lringthevolumeupto 1/2. 1/5 and lflOof theoriginalvirus flnidxeseDtivelyand vaccines were then pxererl. Result indicated that rabbit injctl with the vaccines of 1/5 and 1/10 of the original virus fluid pep3red from the BEI-inactivati and ultracentrifugated antigens suririval afr challenge.