Ri]cettsiales were isolatl from the s extomiad normal cattle after an incculation cfthesmpleUocdofasuspetolRikettsialous cattleinTaipeiHsien.
The Ucxid obiner1 from the incculated cattle was again used to incculated cattle showed fe’r (40-41, joundice and anemia. The numleis of red blcod cells (RBQ dropped doi to bolow 3 millionshril followed by weakness and emaciation. This pathsogen was identified in the ANaplasma marginale (A. marginale) bused on it morphology in REC and serological t.
The Ucxid ctollatal from the inoculated cattle were lysed and the perasial were coll. Antigen for complement-fixation stt were prepared by sonication of pathpgen were high when the antigne was tt by the comparison of it reaction to both standard serum and serum from abiral infa& cattle.
The pathogens were collat4d from the blocd of experimental inoculation of splenODtOmil cattle with A margiriale and conentrated by cent-ifugation when the concentation of pethogens were 10-4-1 0-Shnl in the Uocc. To part ofcoicentraal pathogens the phccphate buffered gbose with 10% normal bovine serum was added and the living immunogen was made. In the other hand the inactivated immunogen was prepared by adding DEAB-dextan into the rest part of pathogens inactivati. by glutaraldehyde. Either one of immunogens prepared in this manner was used to immuni2e 6 month-andhr 1 .5-year-okl cattle. The result indica1 that the living immunogen has a superior capacity of prcducing CF antibody to that of inactivati ummunogen as well as the Cf antibody manitained longer.