A Uocd sample from a dairy farm in Northern Taiwan was incculated in a splnerotorni1 calf; from which the orgainism of Theileria was suoxssfufly isolaS The infeced hlcxA was then transferred to another splenectomii calf and caused it a mild clinical course with the decreased numler of red blood cells (350-450x 1 0”4hnm). The isolae was similar to Theileria sergenti according to it share variation in the blood stream.
A spleneotomiI calf of 3-4 months old was artifically incxou1ad with the iso1a andblolwhen 10% of us Uocdwiththeorganisms. ThecolleciUocdwas hemo1y cxincentated for the organisms. Aft homogenization and sonication, CF antigen was prepared and evaluated, This antigen had a tier of x 8 against the serum of a suffered cae. When it was applied ot deeot CF antibody production on an exrerimental cattle at innervals ofZ 4,8,12 and 16 a post infection, different levelsofantibcdytierofx0,xS,x20,x40andx2oweremeasured. Inthefield,203 cases were assayed with this antigen. The results showed that 36.6-78.2% of them were positive. AnotherlO clinically suffered cattle were also confirmed positive in serum reaction with the antigen. The a&ve facts indicaed the prepared CF antigen was quie sthficandalsopttedonttheexistinceofthis diseaseinTaiwancattle.