Bordetella bronchiseptica phase I strain was used for atrophic rhinitis vaccine production. The bacteria were cultured on potato infusion agar at 37? for 24 ho - urs, harvested and adjusted to a final concentration of 6 x CFU: ml. then ma - ctiveted with formalin and supplemented with prescrvative thimerosal and adjuvant aluminum hydroxide geL
Safety tests were carried out in prernant sows with two kinds of vaccination prozram. one with high doses, i. e. 1 0m1 for basic vaccination and 20 ml for reva - ccination at two weeks interval, the other with designated dose. i. e. 2 ml for 1st vaccination and 5 ml for booster. No clinical sign or abortion was observed in ei - ther group of vaccinated sows. Piglets from the vaccinated sows had maternal antibodies of 1: 1.250-1 :2.560at4-day-old.andl: SOatl2-week-old.A.R.antibodyfree Summary pidets. 5 - week - old, and adult rabbits were also vaccinated with the bacterin. 1 ml at 1st vaccination and 2 ml at revaccination for piclets. and 0.5 ml 1st vaccination 1 ml revaccination for rabbits. No clinical signs was noticed during the experiment period. The average tube agglutination antibody titer was 1: 640 for the vaccinated piglets. and 1:2,560 for rabbits.
In the field trials. 151 of pregnant sows and 157 of rabbits were vaccinated with the bacterin as designated program. Serum samples, 56 from vaccinated sews and 112 from their piglets (5 - day - old) were collected The tube agglutination an- tibody levels were 1: 320 - I : 2,560. for both sows and their piglets. The antib - ody titers of the vaccinated rabbits were 1: 320- 1: 5,120. Immune response cou - Id be induced in sews or rabbits even with low level of AR antibody before vacci - nation.