The 70 positive reacted birds were colkted from lccal poultry farms on which the rericdical examination for Pullonim disease had being rerformed by rapid wholeblocd agglutination test using Pullorum sinerJ K antigen prepared in this Jnstitute. They were first retested with the US, Japan and domestic made antigens as a acomparative study of the three antigens, and lastly tempting isolation of S. pullorum were made. The resulis obtainer! are summari-1 as follows:
1.ithe 70 p€itive birds retesJ by rapid whole blocd methori, 32 birds (43.7%) 33 birds (47,1%) and 44 birds (62.9%)were reactel with the domestic Japan or US made Pullorum antigens resatively. That is, 22 birds (31.4%) showed c€itive aonsforanofthethreantigens,andl3birds (18.5%)for2ldndsandllbirds (24.3%) foronlyineltindof theantigens, while 18 birds (25.7%) showednegative reactions for either antigen.
2.of the 70 pceitive birds retested by the tube-serum method, 23 birds (329%), 19 birds (27.1%)and35 birds (50%)werepcthtive Q50x)byusingthethreeantigens rescaztively.Thatis, l2birds (lZl%),9birds (12,9%) and23 birds (32.9%) were gDSitive for 3, 2or only one kind of the3 antigens, while 26birrls (37.1%) were negative to any of the antigens.
3.The sewn of the 70 positive reactors, only 16 Cases (22.9%) showed positive by agar gel preroipitation test.
4.The definite lesions for Thillowm disease on organs were found in 63 birds (%%) among the 70 positive reactors. The most common finding among those birds was rnissharn, discolored ovary, black-greenish or hownish ovary, pale colored liver and swollen bile dn et.
5.Among the 70 positive birds, S. pullornn organisms were siessfully isolal from 44 birds Theisolationrate was 62.9%.
6.The isolation of S. pullorum was also cerformalon their eggs totally 162 eggs, and only 17 eggs of then-i (10.5%) were positive. That is only from 11 birds eggs (4.4%) weresuccessfullyisolatelwithS. pullorum. Allofthe55 stiins isolatedfromthe Positive birds organs or their eggs were indentified to be S. pullorum by biological characteristics and serolo-gjcal test.