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Update Date: [2013-11-04]

NO.: 8

AHRI report No.08


Adaptation of Lainized Hog Cholera Virus in Rabbit Kidney Cell Culture by assing through the Bridging Cells (Swine Testis Cells)


Department Taiwan Animal Health Researdi Institute
Author IF. Su;T.C, Liii

Regarding so the early studies of Hog Cholera Vtine, many investigators jeid more attention to the inactivated vaccine. Later, they were interested in the development of the living vaccine. In addition, Ircatse of the prompt improvement of the tissue culture terhnique. most of them engaged in studying living vaccines by emplayung the tissue culture hnique. The authors sngly felt that applying tissue culture hnirpae in preration of hog cholera livir*g vaccine was aix impciant work. So we tried to adapt the Upini Virus, which has kem island-widly applied for hog cholera control, in Rabbit Kidney (RK) cell cultures, By which the tissue culture tzhnique could ke emplol for the preeration of living hog cholera vaccine with the newly adapted stain in this island. The result vie are summaril as follows:

1.The Lzpini2rd. Hog Cholera Virus could Ire successfully adapted in Rabbit Kidney (RK) aft seveM passages of proegating the virus in Swine Testis (SI) cell culture and in RK cell culture by turns. Being passed 20 ssages through RK cell culture, no cytoethic effi (CPE) was found in hoset cell.

2. This adapted virus had extemely high immunity pc4ency and pDst-vathmaton reactions were safe. Pig kafozJ DOse (PflJ) reached to 10^3.

3. It was easily deeztal by means of Flourescent Antibcdy Cell Culture Tohniqued 2- st-eptedmethcd. Virus titer (TCEJSO) reachalto 10^3. 
4.The adapted virus mauninal the same fever reaction of lapinized hog cholera virus when injJ to rabbit InfertedDose (ID)inrabbit was reachalto 10^3.



Swine Testis Cells Rabbit Kidney Cell$og Cholera Vaccine