The large scale chicken raising farms in Taiwan have keen sporabiic okeerval with an acute infectious fatal liver disease since February, 1976, The affected chickens died sddenly, The Cowdry type A and Scxtf full intranuclear inclusion body cxowuld ke observed from the aff&oti liver.
he virus has keen successfully isolai from the affected liver by the incxoulation of thicken kidney cell culture and the yolk sac of 6-7 day-old thicken embryo.
The bicchemical properties of isolated virus are: The prcduction of characteristic cytopathic effect (rounding of cells) and the plaque formation in the CK cell culture aft 7-9 days of incculation. Pc€itive pathogenicity to the infected chicken ernbrycs with the lesion of hemorrhage, edema, dwarfing and hepatic narc€is. and production of pcck on the choxicellantoic membrance. No hernagglutination of red blood cells of various species.
The biophysical properties of the virus are resistant to 20% etyhi ether, 0.1% sodium desoxycholate, 0.25% trypsin, pH 3.0 and 1 M?MgQ2 heating for 1 hour. The virus canss through lCOnmMillipoaefilterbutnot50nmfilt Thevirus is DNAvirus (treatment of 50ug/ml IUDR).
The isolal viruses are pathogenic to young chicken (4 day-old). From the abve result, it was suggesJ that the Inclurion Body Hepatitis (IBH) caused by Menovirus was similar to that the first report of this disease by Hernboildt and Frazier.