Adirect immunoperoxidase technique for the identification of immunoglobulincontaining cells in the section of chicken spleen is described. This is applicable to formalin fixed, paraff in- embedded material. It is based on an immunohistochemical method using peroxidase-labelled antibodies.
Rabbits were immunized by purified chicken IgG. The IgO fraction of rabbit anti -chicken Ig 0 antiserum was conjugated with horseradish peroxidase. The presence of immunoglobulinSinninary containing cells in chicken spleen sections was revealed by
staining the tissue-bound peroxidase-labelled antibody with diaminobenzidine, than briefly counterstained with hematoxylin. The endogeneous peroxidase was satisfactorily inhibited by treating the dewaxed section with a fresh 0.5% solution of hydrogen peroxide in absolute methanol for 20 minutes. The structure of immunoglobulin-containing cells in chicken spleen sections is clear, preparations are permanent, and retrospective studies of stored paraffinembedded tissue are possible.