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Publication Simultaneous Determination of Water Soluble Vitamins (B1、B2、B6、Niaci...  

Update Date: [2013-11-13]

NO.: 31
 
Reportno.

AHRI report No.31

Topic

Simultaneous Determination of Water Soluble Vitamins (B1、B2、B6、Niacin) by High Performance Liquid Chromatography in Premixes
 

Department

TawnAnirnal Health Research Institute The Branch Institute of Animal Drugs epartanen Inspection

Author KM. Lin9vLC. Uu.Y. Un
Summary

Premixes were added directly with mobile phase to a certain volume, Alter mixing and filtration, the filirate was inDtd into a high performance liquid chromatography (HPLQ which was equipped with a BECKMAN OCTAL Si-i 4.6x250 mm column. The compc6ition of mobile phase was the mixture of 5 mM PICBS solution (p11
to3.25withaceticxid)andMethanolintheratioof7(I3Ochanginginto 75:25. The peaks of the four vitamins were separated ketter as the ratio of 5 mM PICBS solution was gradually increased in quantify. The cleeztion sensitivity of VinE l?B6, and Niacin by using ogram changeable JJV detector (CI—3.8, 20) nm,3,8—4 1,2% nm,4 .8—GAY, 246 nm,60—7.O’, 266 rim) was higer than that by using fixed wavelength at 275 rim, while it was unchanged for Vit.B2.
 

Keyword

Water soluble vitarnins5’xernix$igh Performance liquid Chromatography.